Scientific applications of the ID02 beamline can be broadly divided into three domains; (1) soft condensed matter, (2) noncrystalline structural biology, and (3) interdisciplinary area of soft matter, biology, and nanoscience.

  • Soft condensed matter

    Soft matter studies most often involve probing the equilibrium, nonequilibrium and transient microstructures in systems such as colloids, polymers, surfactants membranes, proteins, etc. In these studies, a variety of techniques such as rheology, stopped-flow rapid mixing, etc. are combined with SAXS or simultaneous SAXS/WAXS. The high resolution and improved sensitivity of SAXS can be exploited to probe the spatial distribution of counterions in charged soft matter by the anomalous SAXS method. The high dynamic range of SAXS/USAXS techniques permits elucidating the multiple structural levels of a large variety of soft matter systems.

  • Non-crystalline structural biology

    The noncrystalline structural biology work is largely centered around the structure-function relationship. For example in muscle by combining very precise mechanics and high resolution small-angle diffraction allow molecular level understanding of the mechanism of contraction down to the sub-millisecond time scale. Time resolved studies can also be done in solution e.g. by combining with the stopped-flow mixing to probe the supermolecular structure under in-vitro conditions. Examples include protein interactions and folding.

  • Interdisciplinary areas of soft matter and nanoscience

    Many exotic nanostructured systems are realized by the hierarchical self-assembly of complex molecules. High resolution SAXS or a combination of SAXS and WAXS is a very powerful and non destructive technique to elucidate such hierarchical structures. Examples include the self-assembly of amphiphilic peptides, complexes of cationic lipids with biopolymers such as DNA, actin, etc. In another situation, the growth kinetics of pyrolytic nanoparticles under extreme dilute conditions (φ < 10-7) can be studied in the sub-millisecond range.

  • Industrial

    Combined SAXS/WAXS is a powerful method to determine the microstructure and phase behavior of multi-component systems involved in cosmetics, detergents, pharmaceuticals, polymers, etc. In addition, in-situ studies can be performed under similar conditions as that involved in industrial processing (e.g. polymers).

 

Selected examples:

 

 

 

 

Further Information