BM29 BioSAXS

Synopsis

The BioSAXS beamline is a highly automated beamline dedicated to the study of proteins, macromolecular complexes, viruses etc., in solution. Samples can be investigated under various conditions (temperature, buffer, pH, kinetics) in a high-throughput manner or a HPLC unit can be used for in-situ (online) purification.
Status:  open

Disciplines

  • Life Sciences
  • Chemistry
  • Medicine

Applications

  • Structural biology
  • Pharmaceuticals

Techniques

  • SAXS - small-angle X-ray scattering

Energy range

  • 7.0 - 15.0  keV

Beam size

  • Minimum (H x V) : 50.0 x 50.0  µm²
  • Maximum (H x V) : 2.0 x 1.0  mm²

Sample environments

  • Quartz capillary as a part of automated sample changer allowing temperature variations (from 4 to 60°C)
  • HPLC (high performance liquid chromatography) system can be used in parallel with sample changer

Detectors

  • Pilatus 1M

Pernot P., Theveneau P., Giraud T., Nogueira Fernandes R., Nurizzo D., Spruce D., Surr J., McSweeney S., Round A., Felisaz F., Foedinger L., Gobbo A., Huet J., Villard C. and Cipriani F., "New beamline dedicated to solution scattering from biological macromolecules at the ESRF", Journal of Physics: Conference Series 247 (2010) 012009-1-012009-8.