Baldassarri E.J., Ortore M.G., Spinozzi F., Round A., Ferrero C., Mariani P.,
Nanomaterials 10, 629-1-629-20 (2020)
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BM29 BioSAXS
Synopsis
The BioSAXS beamline is a highly automated beamline dedicated to the study of proteins, macromolecular complexes, viruses etc., in solution. Samples can be investigated under various conditions (temperature, buffer, pH, kinetics) in a high-throughput manner or a HPLC unit can be used for in-situ (online) purification.
Status:
open
Disciplines
- Life Sciences
- Chemistry
- Medicine
Applications
- Structural biology
- Pharmaceuticals
Techniques
- BioSAXS - small-angle X-ray scattering (proteins/DNA)
- SAXS - small-angle X-ray scattering
Energy range
- 7.0 - 15.0 keV
Beam size
- Minimum (H x V) : 50.0 x 50.0 µm²
- Maximum (H x V) : 2.0 x 1.0 mm²
Sample environments
- Quartz capillary as a part of automated sample changer allowing temperature variations (from 4 to 60°C)
- HPLC (high performance liquid chromatography) system can be used in parallel with sample changer
Detectors
- Pilatus3 2M in-vac
Pernot P., Theveneau P., Giraud T., Nogueira Fernandes R., Nurizzo D., Spruce D., Surr J., McSweeney S., Round A., Felisaz F., Foedinger L., Gobbo A., Huet J., Villard C. and Cipriani F., "New beamline dedicated to solution scattering from biological macromolecules at the ESRF", Journal of Physics: Conference Series 247 (2010) 012009-1-012009-8.
Latest publications
View all publications
Bertrand Q., Job V., Maillard A.P., Imbert L., Teulon J., Favier A., Pellequer J.L., Huber P., Attrée I., Dessen A.,
Journal of Molecular Biology 432, 4466-4480 (2020)
Bonchuk A., Kamalyan S., Mariasina S., Boyko K., Popov V., Maksimenko O., Georgiev P.,
Scientific Reports 10, 2677-1-2677-11 (2020)
Choukate K., Gupta A., Basu B., Virk K., Ganguli M., Chaudhuri B.,
Journal of Structural Biology 209, 107429-1-107429-10 (2020)
Di Gioacchino M., Bianconi A., Burghammer M., Ciasca G., Bruni F., Campi G.,
Biochimica et Biophysica Acta (BBA) - Biomembranes 1862, 183256-1-183256-10 (2020)
Duarte D.P., Lamontanara A.J., La Sala G., Jeong S., Sohn Y.K., Panjkovich A., Georgeon S., Kükenshöner T., Marcaida M.J., Pojer F., De Vivo M., Svergun D., Kim H.S., Dal Peraro M., Hantschel O.,
Nature Communications 11, 2319-1-2319-15 (2020)
partners
European Synchrotron Radiation Facility - 71, avenue des Martyrs, CS 40220, 38043 Grenoble Cedex 9, France.