Structural insights into biological macromolecules by Single particle Electron Microscopy
For decades, single particle electron microscopy (EM) has been providing a wealth of medium to low resolution structures of biological macromolecules. Recent improvements in electron microscopes and detectors have expanded the resolution limits to below 3A. The acquired knowledge is unprecedented providing deeper insights into various biological processes of the cell that were impossible to decipher using existing structural techniques. Albeit the success of cryo-EM, single particle EM using negative staining is still a prevalent technique for small (< 150kDa), heterogenous samples. In this line, I will present my results starting from structures obtained by negative stain EM that provided helpful functional insights on Protein-2C from Poliovirus and a molecular decoy from Orf Virus that mimics cytokines of the human immune system. I will continue with my work on various cryo-EM projects, wherein the resolution undulates with the development in the field, including Hepatitis B virus capsid and more recent results on bacterial lysine decarboxylases.
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