ID30B / MAD

Techniques

MX - macromolecular crystallography

MAD - multi-wavelength anomalous dispersion

Energy range

6.0 - 20.0 keV

Beam size

Minimum (H x V) : 20.0 x 20.0 µm²
Maximum (H x V) : 200.0 x 200.0 µm²

Sample environments

ID30B is equipped with a modified microdiffractometer (MD2-S), allowing data collection from micron-sized crystals mounted on conventional cryo-supports or in crystallisation plates (in situ).

A new generation, flexible and high throughput sample changer (FlexHCD) capable of transferring samples from either EMBL/ESRF pucks or Unipucks has also been developed for ID30B in a collaboration between the ESRF and EMBL-Grenoble.

Detectors

PILATUS3 6M 1000 µm Si sensor (Dectris). This detector was funded with support from the French Infrastructure for Integrated Structural Biology (FRISBI) ANR-10-INBS-05 to the CNRS and the ESRF.

AXAS-A X-ray fluorescence detector (KETEK)

McCarthy, A. A., Barrett, R., Beteva, A., Caserotto, H., Dobias, F., Felisaz, F., Giraud, T., Guijarro, M., Janocha, R., Khadrouche, A., Lentini, M., Leonard, G. A., Lopez Marrero, M., Malbet-Monaco, S., McSweeney, S., Nurizzo, D., Papp, G., Rossi, C., Sinoir, J., Sorez, C., Surr, J., Svensson, O., Zander, U., Cipriani, F., Theveneau, P., Mueller-Dieckmann, C. ID30B - a versatile beamline for macromolecular crystallography experiments at the ESRF. J. Synchrotron Radiat. 25,1249-1260 (2018).

Mueller-Dieckmann, C., Bowler, M. W., Carpentier, P., Flot, D., McCarthy, A. A., Nanao, M. H., Nurizzo, D., Pernot, P., Popov, A., Round, A., Royant, A., de Sanctis, D., von Stetten, D., Leonard, G. A. The status of the macromolecular crystallography beamlines at the European Synchrotron Radiation Facility, Eur. Phys. J. Plus 130, 70 (2015).

Crystal structure and functional characterization of selenocysteine-containing glutathione peroxidase 4 suggests an alternative mechanism of peroxide reduction

Borchert A., Kalms J., Roth S.R., Rademacher M., Schmidt A., Holzhutter H.G., Kuhn H., Scheerer P.,
Biochimica et Biophysica Acta 1863, 1095-1107 (2018)

Lytic xylan oxidases from wood-decay fungi unlock biomass degradation

Couturier M., Ladevèze S., Sulzenbacher G., Ciano L., Fanuel M., Moreau C., Villares A., Cathala B., Chaspoul F., Frandsen K.E., Labourel A., Herpoel-Gimbert I., Grisel S., Haon M., Lenfant N., Rogniaux H., Ropartz D., Davies G.J., Rosso M.N., Walton P.H., Henrissat B., Berrin J.G.,
Nature Chemical Biology 14, 306-310 (2018)

Neuropathic MORC2 mutations perturb GHKL ATPase dimerization dynamics and epigenetic silencing by multiple structural mechanisms

Douse C.H., Bloor S., Liu Y., Shamin M., Tchasovnikarova I.A., Timms R.T., Lehner P.J., Modis Y.,
Nature Communications 9, 651-1-651-15 (2018)

Porin self-association enables cell-to-cell contact in Providencia stuartii floating communities

El-Khatib M., Nasrallah C., Lopes J., Tran Q.T., Tetreau G., Basbous H., Fenel D., Gallet B., Lethier M., Bolla J.M., Pagès J.M., Vivaudou M., Weik M., Winterhalter M., Colletier J.P.,
Proceedings of the National Academy of Sciences of the USA 115, E2220-E2228 (2018)

A novel bacterial class V dye-decolourizing peroxidase from the extremophile Deinococcus radiodurans: Cloning, expression optimization, purification, crystallization, initial characterization and X-ray diffraction analysis

Frade K.S.T., Fernandes A.C.P., Silveira C.M., Frazão C., Moe E.,
Acta Crystallographica F 74, 419-424 (2018)

Discovery and characterization of a thermostable two-domain GH6 endoglucanase from a compost metagenome

Jensen M.S., Fredriksen L., MacKenzie A.K., Pope P.B., Leiros I., Chylenski P., Williamson A.K., Christopeit T., Ostby H., Vaaje-Kolstad G., Eijsink V.G.H.,
PloS One 13, e0197862-1-e0197862-22 (2018)

ID30B / MAD

Synopsis

Disciplines

Applications

Techniques

Energy range

Beam size

Sample environments

Detectors

Latest publications