Kampatsikas I., Bijelic A., Pretzler M., Rompel A.,
Angewandte Chemie International Edition 58, 7475-7479 (2019)
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- ID29 MX tunable microbeam
ID29 MX tunable microbeam
Synopsis
ID29 is a fully automated macromolecular crystallography beamline, intended for high-energy-resolution anomalous dispersion phasing experiments and for high-resolution X-ray diffraction experiments.
Status:
open
Disciplines
- Life Sciences
Applications
- Life science
- Biological science
- Biochemical science
Techniques
- MX - macromolecular crystallography
- MAD - multi-wavelength anomalous dispersion
Energy range
- 6.0 - 20.0 keV
Beam size
- Minimum (H x V) : 10.0 x 10.0 µm²
- Maximum (H x V) : 40.0 x 30.0 µm²
Sample environments
- 100 K cryostream
- Automatic sample changer
- Mini-kappa goniometer
- Dehydration device
- Pressurised cell
Detectors
- Pilatus 6M (Dectris)
- Rontec Xflash X-ray fluorescence detector
Technical details
ID29 is intended for high intensity, high energy resolution MAD and SAD measurements over a wide range of X-ray wavelengths (0.7 - 2.1 Å). ID29 is equipped with a MicroDiffractometer (MD2) - which allows to tailor the beam sizes down to 75 (full beam), 50, 30, 20 and 10 microns in diameter - and with a FlexHCD Sample Changer. Diffraction data are recorded with a fast readout Pilatus 6M-F pixel detector detector (Dectris LTD) that allows data collection with a maximum frame rate of 25 images/s.
Latest publications
View all publications
Pabis M., Popowicz G., Stehle R., Fernandez-Ramos D., Asami S., Warner L., Garcia-Maurino S., Schlundt A., Martinez-Chantar M., Díaz-Moreno I., Sattler M.,
Nucleic Acids Research 47, 1011-1029 (2019)
Parkash V., Kulkarni Y., ter Beek J., Shcherbakova P.V., Kamerlin S.C.L., Johansson E.,
Nature Communications 10, 373-1-373-13 (2019)
Xin B.T., Huber E.M., de Bruin G., Heinemeyer W., Maurits E., Espinal C., Du Y.M., Janssens M., Weyburne E.S., Kisselev A.F., Florea B.I., Driessen C., van der Marel G.A., Groll M., Overkleeft H.S.,
Journal of Medicinal Chemistry 62, 1626-1642 (2019)
Chérier D.
From: Université Paris-Saclay, France (PhD Thesis),
2017
Di Méo T.
From: Université Paris-Saclay, France (PhD Thesis),
2017
partners
European Synchrotron Radiation Facility - 71, avenue des Martyrs, CS 40220, 38043 Grenoble Cedex 9, France.