Dabin J.
From: Université Pierre et Marie Curie, France (PhD Thesis),
2088
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- ID29 MX tunable microbeam
ID29 MX tunable microbeam
Synopsis
ID29 is a fully automated macromolecular crystallography beamline, intended for high-energy-resolution anomalous dispersion phasing experiments and for high-resolution X-ray diffraction experiments.
Status:
open
Disciplines
- Life Sciences
Applications
- Life science
- Biological science
- Biochemical science
Techniques
- MX - macromolecular crystallography
- MAD - multi-wavelength anomalous dispersion
Energy range
- 6.0 - 20.0 keV
Beam size
- Minimum (H x V) : 10.0 x 10.0 µm²
- Maximum (H x V) : 75.0 x 30.0 µm²
Sample environments
- 100 K cryostream
- Automatic sample changer
- Mini-kappa goniometer
- Dehydration device
- Pressurised cell
Detectors
- Pilatus 6M (Dectris)
- Rontec Xflash X-ray fluorescence detector
Technical details
ID29 is intended for high intensity, high energy resolution MAD and SAD measurements over a wide range of X-ray wavelengths (0.7 - 2.1 Å). ID29 is equipped with a MicroDiffractometer (MD2) - which allows to tailor the beam sizes down to 75 (full beam), 50, 30, 20 and 10 microns in diameter - and with a EMBL/ESRF Sample Changer. Diffraction data are recorded with a fast readout Pilatus 6M pixel detector detector (Dectris LTD) that allows data collection with a maximum frame rate of 12 images/s.
Latest publications
View all publications
Bai Y., Gangoiti J., Dijkstra B.W., Dijkhuizen L., Pijning T.,
Structure 25, 231-242 (2017)
Bassler J., Ahmed Y.L., Kallas M., Kornprobst M., Calviño F.R., Gnädig M., Thoms M., Stier G., Ismail S., Kharde S., Castillo N., Griesel S., Bastuck S., Bradatsch B., Thomson E., Flemming D., Sinning I., Hurt E.,
Protein Science 26, 327-342 (2017)
Blondiaux N., Moune M., Desroses M., Frita R., Flipo M., Mathys V., Soetaert K., Kiass M., Delorme V., Djaout K., Trebosc V., Kemmer C., Wintjens R., Wohlkönig A., Antoine R., Huot L., Hot D., Coscolla M., Feldmann J., Gagneux S., Locht C., Brodin P., Gitzinger M., Déprez B., Willand N., Baulard A.R.,
Science 355, 1206-1211 (2017)
Bock T., Volz C., Hering V., Scrima A., Müller R., Blankenfeldt W.,
Nucleic Acids Research 45, 2166-2178 (2017)
Bonnet J., Cartannaz J., Tourcier G., Contreras-Martel C., Kleman J.P., Morlot C., Vernet T., Di Guilmi A.M.,
Scientific Reports 7, 43564-1-43564-14 (2017)
partners
European Synchrotron Radiation Facility - 71, avenue des Martyrs, CS 40220, 38043 Grenoble Cedex 9, France.