ID29 MX tunable microbeam

Synopsis

ID29 is a fully automated macromolecular crystallography beamline, intended for high-energy-resolution anomalous dispersion phasing experiments and for high-resolution X-ray diffraction experiments.
Status:  open

Disciplines

  • Life Sciences

Applications

  • Life science
  • Biological science
  • Biochemical science

Techniques

  • MX - macromolecular crystallography
  • MAD - multi-wavelength anomalous dispersion

Energy range

  • 6.0 - 20.0  keV

Beam size

  • Minimum (H x V) : 10.0 x 10.0  µm²
  • Maximum (H x V) : 75.0 x 30.0  µm²

Sample environments

  • 100 K cryostream
  • Automatic sample changer
  • Mini-kappa goniometer
  • Dehydration device
  • Pressurised cell

Detectors

  • Pilatus 6M (Dectris)
  • Rontec Xflash X-ray fluorescence detector

Technical details

ID29 is intended for high intensity, high energy resolution MAD and SAD measurements over a wide range of X-ray wavelengths (0.7 - 2.1 Å). ID29 is equipped with a MicroDiffractometer (MD2) - which allows to tailor the beam sizes down to 75 (full beam), 50, 30, 20 and 10 microns in diameter - and with a EMBL/ESRF Sample Changer. Diffraction data are recorded with a fast readout Pilatus 6M pixel detector detector (Dectris LTD) that allows data collection with a maximum frame rate of 12 images/s.

Latest publications

View all publications
Carba-cyclophellitols are neutral retaining-glucosidase inhibitors

Beenakker T.J.M., Wander D.P.A., Offen W.A., Artola M., Raich L., Ferraz M.J., Li K.Y., Houben J.H.P.M., van Rijssel E.R., Hansen T., van der Marel G.A., Codee J.D.C., Aerts J.M.F.G., Rovira C., Davies G.J., Overkleeft H.S.,
Journal of the American Chemical Society 139, 6534-6537 (2017)