ID30A-3 / MASSIF-3

Applications

MASSIF-3 is a microfocus beamline (15 µm diameter), operating at fixed energy (12.81 keV). The beamline is dedicated to protein crystals of small size, inhomogeneous protein crystals, and fast data collection, and is also suitable for SAD measurements (Se, Pt, Au,...). MASSIF-3 is equipped with a MD2 micro-diffractometer and with a FlexHCD sample changer for SPINE pucks and UniPucks. Diffraction data are recorded on a Eiger X 4M detector that allows data collection at a very high frame rate (up to 750 Hz).

Techniques

Anomalous diffraction

MX - macromolecular crystallography

Microbeam

Microcrystallography

Optical spectroscopy

Single-crystal diffraction

XRD - X-ray diffraction

Energy range

12.81 - 12.81 keV

Beam size

Minimum (H x V) : 15.0 x 15.0 µm²
Maximum (H x V) : 15.0 x 15.0 µm²

Detectors

Eiger X 4M

The Upgrade Programme for the Structural Biology beamlines at the European Synchrotron Radiation Facility – High throughput sample evaluation and automation, P. Theveneau et al. (2013) J. Phys.: Conf. Ser. 425 012001 doi:10.1088/1742-6596/425/1/012001

Structure analysis of thymidylate synthase from white spot syndrome virus reveals WSSV-specific structural elements

Panchal V., Kumar S., Hossain S.N., Vasudevan D.,
International Journal of Biological Macromolecules 167, 1168-1175 (2021)

Characterization of the stereoselective P450 enzyme BotCYP enables the in vitro biosynthesis of the bottromycin core scaffold

Adam S., Franz L., Milhim M., Bernhardt R., Kalinina O.V., Koehnke J.,
Journal of the American Chemical Society 142, 20560-20565 (2020)

Millisecond time-resolved serial oscillation crystallography of a blue-light photoreceptor at a synchrotron

Aumonier S., Santoni G., Gotthard G., von Stetten D., Leonard G.A., Royant A.,
IUCrJ 7, 728-736 (2020)

Methionine-rich loop of multicopper oxidase McoA follows open-to-close transitions with a role in enzyme catalysis

Borges P.T., Brissos V., Hernandez G., Masgrau L., Lucas M.F., Monza E., Frazão C., Cordeiro T.N., Martins L.O.,
ACS Catalysis 10, 7162-7176 (2020)

Structure and catalytic regulation of Plasmodium falciparum IMP specific nucleotidase

Carrique L., Ballut L., Shukla A., Varma N., Ravi R., Violot S., Srinivasan B., Ganeshappa U.T., Kulkarni S., Balaram H., Aghajari N.,
Nature Communications 11, 3228-1-3228-11 (2020)

3-Oxo-ß-sultam as a sulfonylating chemotype for inhibition of serine hydrolases and activity-based protein profiling

Carvalho L.A.R., Almeida V.T., Brito J.A., Lum K.M., Oliveira T.F., Guedes R.C., Goncalves L.M., Lucas S.D., Cravatt B.F., Archer M., Moreira R.,
ACS Chemical Biology 15, 878-883 (2020)

ID30A-3 / MASSIF-3

Synopsis

Disciplines

Applications

Techniques

Energy range

Beam size

Sample environments

Detectors

Latest publications