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Synopsis
Note that MASSIF-3 is not a fully automatic beamline (unlike MASSIF-1) and is operated by local or remote users (like ID29 and ID23-1/2).
Latest news: Since Feb. 5th 2016, an Eiger 4M detector has been installed on MASSIF-3, which can record up to 750 images per second. Note that the recorded images are in the HDF5 format.
The MASSIF (Massively Automated Sample Selection Integrated Facility) suite of beam lines comprises three individual end-stations, MASSIF-1, MASSIF-2 and MASSIF-3, all located at the first branch of ID30. All three beam lines run at a fixed photon energy slightly above the selenium absorption edge.
The third endstation MASSIF-3, in user operation since December 2014, offers a small X-ray beam (15 µm diameter) at a fixed energy (12.8 keV), and is dedicated to perform experiments with very small protein crystals. MASSIF-3 is equipped with a Eiger X 4M and a mini-diffractomer and a SC3 sample changer.
The optical design of MASSIF-3 makes use of a horizontally diffraction Si (111) monochromator, and compound refractive lenses (CRLs) and a half-Kirkpatrick-Baez (KB) geometry as the vertical and horizontal focusing systems, respectively. The X-ray sources for the three stations are canted undulators mounted on a special carriage on the storage ring. The MASSIF-3 undulator is a U21.2. It is a pseudo-monochromatic source with a single first harmonic at 12.9 keV. An overview of the setup of the optical and experimental hutches can be found here.
Scientific Applications
Protein crystal samples are becoming ever smaller, with today's samples being typically around 10 to 100 microns in size. MASSIF-3 offers a very intense focused beam of 15 µm in diameter (FWHM) to allow to quickly analyze protein microcrystals, using advanced features like mesh scans and X-ray centring. The possibility to additionally record fluorescence and UV/Vis absorption spectra makes MASSIF-3 a great tool for sample evaluation.
Available Techniques
- microfocus beam
- fixed wavelength rapid data collection (SAD/MIR)
- microspectrophotometer available upon request
- HC1 humidity control device available upon request