Biterova E., Ignatyev A., Uusimaa J., Hinttala R., Ruddock L.W.,
PloS One 13, e0202391-1-e0202391-20 (2018)
- Home
- Users & Science
- Find a beamline
- Structural biology
- Our beamlines
- ID30A-3 / MASSIF-3
ID30A-3 / MASSIF-3
Synopsis
Fixed energy microfocus beamline
Status:
open
Disciplines
- Life Sciences
Applications
- MASSIF-3 is a microfocus beamline (15 µm diameter), operating at fixed energy (12.81 keV). The beamline is dedicated to protein crystals of small size, inhomogeneous protein crystals, and fast data collection, and is also suitable for SAD measurements (Se, Pt, Au,...). MASSIF-3 is equipped with a MD2 micro-diffractometer and with a FlexHCD sample changer for SPINE pucks and UniPucks. Diffraction data are recorded on a Eiger X 4M detector that allows data collection at a very high frame rate (up to 750 Hz).
Techniques
- XRD - X-ray diffraction
- Anomalous diffraction
- MX - macromolecular crystallography
- Microbeam
- Microcrystallography
- Single-crystal diffraction
- Optical spectroscopy
Energy range
- 12.81 - 12.81 keV
Beam size
- Minimum (H x V) : 15.0 x 15.0 µm²
- Maximum (H x V) : 15.0 x 15.0 µm²
Sample environments
- 100 K cryostream
- automatic sample changer
- dehydration device
- online microspec
Detectors
- Eiger X 4M
The Upgrade Programme for the Structural Biology beamlines at the European Synchrotron Radiation Facility – High throughput sample evaluation and automation, P. Theveneau et al. (2013) J. Phys.: Conf. Ser. 425 012001 doi:10.1088/1742-6596/425/1/012001
Latest publications
View all publications
Borchert A., Kalms J., Roth S.R., Rademacher M., Schmidt A., Holzhutter H.G., Kuhn H., Scheerer P.,
Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids 1863, 1095-1107 (2018)
Borodkin V.S., Rafie K., Selvan N., Aristotelous T., Navrátilová I., Ferenbach A.T., van Aalten D.M.F.,
ACS Chemical Biology 13, 1353-1360 (2018)
Büttner D., Kramer J.S., Klingler F.M., Wittmann S.K., Hartmann M.R., Kurz C.G., Kohnhäuser D., Weizel L., Brüggerhoff A., Frank D., Steinhilber D., Wichelhaus T.A., Pogoryelov D., Proschak E.,
ACS Infectious Diseases 4, 360-372 (2018)
Carter M., Jemth A.S., Carreras-Puigvert J., Herr P., Martinez Carranza M., Vallin K.S.A., Throup A., Helleday T., Stenmark P.,
Structure 26, 295-303 (2018)
Cotton T.R., Fischer G., Wang X., McCoy J.C., Czepnik M., Thompson T.B., Hyvönen M.,
EMBO Journal 37, 367-383 (2018)
partners
European Synchrotron Radiation Facility - 71, avenue des Martyrs, CS 40220, 38043 Grenoble Cedex 9, France.