ID30A-3 / MASSIF-3

Applications

MASSIF-3 is a microfocus beamline (15 µm diameter), operating at fixed energy (12.81 keV). The beamline is dedicated to protein crystals of small size, inhomogeneous protein crystals, and fast data collection, and is also suitable for SAD measurements (Se, Pt, Au,...). MASSIF-3 is equipped with a MD2 micro-diffractometer and with a FlexHCD sample changer for SPINE pucks and UniPucks. Diffraction data are recorded on a Eiger X 4M detector that allows data collection at a very high frame rate (up to 750 Hz).

Techniques

XRD - X-ray diffraction

Anomalous diffraction

MX - macromolecular crystallography

Microbeam

Microcrystallography

Single-crystal diffraction

Optical spectroscopy

Energy range

12.81 - 12.81 keV

Beam size

Minimum (H x V) : 15.0 x 15.0 µm²
Maximum (H x V) : 15.0 x 15.0 µm²

Detectors

Eiger X 4M

The Upgrade Programme for the Structural Biology beamlines at the European Synchrotron Radiation Facility – High throughput sample evaluation and automation, P. Theveneau et al. (2013) J. Phys.: Conf. Ser. 425 012001 doi:10.1088/1742-6596/425/1/012001

Crystal structure and functional characterization of selenocysteine-containing glutathione peroxidase 4 suggests an alternative mechanism of peroxide reduction

Borchert A., Kalms J., Roth S.R., Rademacher M., Schmidt A., Holzhutter H.G., Kuhn H., Scheerer P.,
Biochimica et Biophysica Acta 1863, 1095-1107 (2018)

O-GlcNAcase fragment discovery with fluorescence polarimetry

Borodkin V.S., Rafie K., Selvan N., Aristotelous T., Navrátilová I., Ferenbach A.T., van Aalten D.M.F.,
ACS Chemical Biology 13, 1353-1360 (2018)

Challenges in the development of a thiol-based broad-spectrum inhibitor for metallo-β-lactamases

Büttner D., Kramer J.S., Klingler F.M., Wittmann S.K., Hartmann M.R., Kurz C.G., Kohnhäuser D., Weizel L., Brüggerhoff A., Frank D., Steinhilber D., Wichelhaus T.A., Pogoryelov D., Proschak E.,
ACS Infectious Diseases 4, 360-372 (2018)

Human NUDT22 Is a UDP-glucose/galactose hydrolase exhibiting a unique structural fold

Carter M., Jemth A.S., Carreras-Puigvert J., Herr P., Martinez Carranza M., Vallin K.S.A., Throup A., Helleday T., Stenmark P.,
Structure 26, 295-303 (2018)

Structure of the human myostatin precursor and determinants of growth factor latency

Cotton T.R., Fischer G., Wang X., McCoy J.C., Czepnik M., Thompson T.B., Hyvönen M.,
EMBO Journal 37, 367-383 (2018)

Structures of Teneurin adhesion receptors reveal an ancient fold for cell-cell interaction

Jackson V.A., Meijer D.H., Carrasquero M., van Bezouwen L.S., Lowe E.D., Kleanthous C., Janssen B.J.C., Seiradake E.,
Nature Communications 9, 1079-1-1079-9 (2018)

ID30A-3 / MASSIF-3

Synopsis

Disciplines

Applications

Techniques

Energy range

Beam size

Sample environments

Detectors

Latest publications