Synopsis
Fixed energy microfocus beamline
Status:
open
Disciplines
Applications
- MASSIF-3 is a microfocus beamline (15 µm diameter), operating at fixed energy (12.81 keV). The beamline is dedicated to protein crystals of small size, inhomogeneous protein crystals, and fast data collection, and is also suitable for SAD measurements (Se, Pt, Au,...). MASSIF-3 is equipped with a MD2 micro-diffractometer and with a FlexHCD sample changer for SPINE pucks and UniPucks. Diffraction data are recorded on a Eiger X 4M detector that allows data collection at a very high frame rate (up to 750 Hz).
Techniques
-
Anomalous diffraction
-
MX - macromolecular crystallography
-
Microbeam
-
Microcrystallography
-
Optical spectroscopy
-
Single-crystal diffraction
-
XRD - X-ray diffraction
Beam size
- Minimum (H x V) : 15.0
x 15.0
µm²
-
Maximum (H x V) : 15.0
x 15.0
µm²
Sample environments
- 100 K cryostream
- automatic sample changer
- dehydration device
- online microspec
Detectors
The Upgrade Programme for the Structural Biology beamlines at the European Synchrotron Radiation Facility – High throughput sample evaluation and automation, P. Theveneau et al. (2013) J. Phys.: Conf. Ser. 425 012001 doi:10.1088/1742-6596/425/1/012001
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Characterization of the stereoselective P450 enzyme BotCYP enables the in vitro biosynthesis of the bottromycin core scaffold
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Structure and catalytic regulation of Plasmodium falciparum IMP specific nucleotidase
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