ID30A-3 / MASSIF-3

Applications

MASSIF-3 is a microfocus beamline (15 µm diameter), operating at fixed energy (12.81 keV). The beamline is dedicated to protein crystals of small size, inhomogeneous protein crystals, and fast data collection, and is also suitable for SAD measurements (Se, Pt, Au,...). MASSIF-3 is equipped with a MD2 micro-diffractometer and with a FlexHCD sample changer for SPINE pucks and UniPucks. Diffraction data are recorded on a Eiger X 4M detector that allows data collection at a very high frame rate (up to 750 Hz).

Techniques

XRD - X-ray diffraction

Anomalous diffraction

MX - macromolecular crystallography

Microbeam

Microcrystallography

Single-crystal diffraction

Optical spectroscopy

Energy range

12.81 - 12.81 keV

Beam size

Minimum (H x V) : 15.0 x 15.0 µm²
Maximum (H x V) : 15.0 x 15.0 µm²

Detectors

Eiger X 4M

The Upgrade Programme for the Structural Biology beamlines at the European Synchrotron Radiation Facility – High throughput sample evaluation and automation, P. Theveneau et al. (2013) J. Phys.: Conf. Ser. 425 012001 doi:10.1088/1742-6596/425/1/012001

Crystal structure of the tubulin tyrosine carboxypeptidase complex VASH1-SVBP

Adamopoulos A., Landskron L., Heidebrecht T., Tsakou F., Bleijerveld O.B., Altelaar M., Nieuwenhuis J., Celie P.H.N., Brummelkamp T.R., Perrakis A.,
Nature Structural & Molecular Biology 26, 567-570 (2019)

Molecular features of steroid-binding antidins and their use for assaying serum progesterone

Agrawal N., Lehtonen S.I., Uusi-Mäkelä M., Jain P., Viitala S., Määttä J.A.E., Kähkönen N., Azizi L., Riihimäki T.A., Kulomaa M.S., Johnson M.S., Hytönen V.P., Airenne T.T.,
PloS One 14, e0212339-1-e0212339-27 (2019)

Architecture of human Rag GTPase heterodimers and their complex with mTORC1

Anandapadamanaban M., Masson G.R., Perisic O., Berndt A., Kaufman J., Johnson C.M., Santhanam B., Rogala K.B., Sabatini D.M., Williams R.L.,
Science 366, 203-210 (2019)

Cristallographie monochromatique résolue en temps au synchrotron d'un domaine de photorécepteur de plante (Time-resolved monochromatic synchrotron crystallography of a plant photoreceptor domain)

Aumonier S.
From: Université Grenoble Alpes, France (PhD thesis), 2019

Inhibitory properties of ATP-competitive coumestrol and boldine are correlated to different modulations of CK2 flexibility

Battistutta R., Lolli G.,
Journal of Natural Products 82, 1014-1018 (2019)

Analysis of a new flavodiiron core structural arrangement in Flv1-ΔFlR protein from Synechocystis sp. PCC6803

Borges P.T., Romão C.V., Saraiva L.M., Goncalves V.L., Carrondo M.A., Teixeira M., Frazão C.,
Journal of Structural Biology 205, 91-102 (2019)

ID30A-3 / MASSIF-3

Synopsis

Disciplines

Applications

Techniques

Energy range

Beam size

Sample environments

Detectors

Latest publications