ID30A-3 / MASSIF-3

Applications

MASSIF-3 is a microfocus beamline (15 µm diameter), operating at fixed energy (12.81 keV). The beamline is dedicated to protein crystals of small size, inhomogeneous protein crystals, and fast data collection, and is also suitable for SAD measurements (Se, Pt, Au,...). MASSIF-3 is equipped with a MD2 micro-diffractometer and with a FlexHCD sample changer for SPINE pucks and UniPucks. Diffraction data are recorded on a Eiger X 4M detector that allows data collection at a very high frame rate (up to 750 Hz).

Techniques

XRD - X-ray diffraction

Anomalous diffraction

MX - macromolecular crystallography

Microbeam

Microcrystallography

Single-crystal diffraction

Optical spectroscopy

Energy range

12.81 - 12.81 keV

Beam size

Minimum (H x V) : 15.0 x 15.0 µm²
Maximum (H x V) : 15.0 x 15.0 µm²

Detectors

Eiger X 4M

The Upgrade Programme for the Structural Biology beamlines at the European Synchrotron Radiation Facility – High throughput sample evaluation and automation, P. Theveneau et al. (2013) J. Phys.: Conf. Ser. 425 012001 doi:10.1088/1742-6596/425/1/012001

Analysis of a new flavodiiron core structural arrangement in Flv1-ΔFlR protein from Synechocystis sp. PCC6803

Borges P.T., Romão C.V., Saraiva L.M., Goncalves V.L., Carrondo M.A., Teixeira M., Frazão C.,
Journal of Structural Biology 205, 91-102 (2019)

The structure of the nucleoprotein of Influenza D shows that all Orthomyxoviridae nucleoproteins have a similar NPCORE, with or without a NPTAIL for nuclear transport

Donchet A., Oliva J., Labaronne A., Tengo L., Miloudi M., Gérard F.C.A., Mas C., Schoehn G., Ruigrok R.W.H., Ducatez M., Crépin T.,
Scientific Reports 9, 600-1-600-14 (2019)

Structure of the SLy1 SAM homodimer reveals a new interface for SAM domain self-association

Kukuk L., Dingley A.J., Granzin J., Nagel-Steger L., Thiagarajan-Rosenkranz P., Ciupka D., Hänel K., Batra-Safferling R., Pacheco V., Stoldt M., Pfeffer K., Beer-Hammer S., Willbold D., Koenig B.W.,
Scientific Reports 9, 54-1-54-14 (2019)

Structural analysis of human NHLRC2, mutations of which are associated with FINCA disease

Biterova E., Ignatyev A., Uusimaa J., Hinttala R., Ruddock L.W.,
PloS One 13, e0202391-1-e0202391-20 (2018)

Crystal structure and functional characterization of selenocysteine-containing glutathione peroxidase 4 suggests an alternative mechanism of peroxide reduction

Borchert A., Kalms J., Roth S.R., Rademacher M., Schmidt A., Holzhutter H.G., Kuhn H., Scheerer P.,
Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids 1863, 1095-1107 (2018)

O-GlcNAcase fragment discovery with fluorescence polarimetry

Borodkin V.S., Rafie K., Selvan N., Aristotelous T., Navrátilová I., Ferenbach A.T., van Aalten D.M.F.,
ACS Chemical Biology 13, 1353-1360 (2018)

ID30A-3 / MASSIF-3

Synopsis

Disciplines

Applications

Techniques

Energy range

Beam size

Sample environments

Detectors

Latest publications