These page contain all the information required to complete a successful experiment at the Macromolecular Crystallography Group beamlines of the ESRF.

Before you come to the ESRF please read the "Prepare Your Experiment" pages. Once here the "Run Your Experiment" pages will help you along the way and if you get into any difficulties the "Trouble Shooting" pages should have the answers. All of our beamlines are equipped with a high precision minidiffractometer and a sample changer (for which you must use SPINE standard pins).

Changer-mini

 

Please cite the paper describing MXCuBE for experiments conducted at the ESRF Structural Biology Beamlines, Gabadinho, J., et al. (2010).MxCuBE: a synchrotron beamline control environment customised for macromolecular crystallography experiments. J. Synch. Rad., 17, 700-707

 

View structures deposited in the Protein Data Bank (PDB) solved using the ESRF MX Beamlines.

Beamline Energy Range Typical Beamsize (microns)  Resolution Limits Typical exposure Time Maximum recommended phi speed  Number of passes Typical transmission for MAD/SAD  

ID23-1
MAD

0.62 to 2.5Å (20 to 5.0keV)

50

0.64Å

0.037 - 0.5 sec

120 degrees/s

1

1-5%

ID23-2
Microfocus

0.873Å (14.200keV)

5x5

1.25Å

0.02 - 0.1 sec

120 degrees/s

1

0.1-15%

ID29
MAD

0.62 to 2.4Å (20 to 5.2keV)

60

0.64Å

0.02 - 0.5 sec

120 degrees/s

1

1-5%

BM29
bio-SAXS

0.83 to 1.77Å (15 to 7 keV)

200

1nm

1 sec

no rotation

 10 frames

Usually 50%

ID30A / MASSIF-1

12.8keV

 100

0.9Å

0.1 - 1 sec

120 degrees/s

1

5-20%
ID30A / MASSIF-2 12.85keV 100 N/A N/A N/A N/A N/A
ID30A / MASSIF-3 12.8keV 15 1.5Å 0.01 sec 120 degrees/s 1 1-5%
ID30B / MAD 2.1 to 0.6Å
(6 to 20keV)		 20-200 0.9Å 0.037 sec 120 degrees/s 1 1-10%