ID30B / MAD

Synopsis

ID30B is a highly automated, undulator-based, tunable beamline dedicated to macromolecular crystallography.
Status:  open

Disciplines

  • Life Sciences

Applications

  • Life sciences
  • Biological Science
  • Biochemistry
  • Structural biology

Techniques

  • MX - macromolecular crystallography
  • MAD - multi-wavelength anomalous dispersion

Energy range

  • 6.0 - 20.0  keV

Beam size

  • Minimum (H x V) : 20.0 x 20.0  µm²
  • Maximum (H x V) : 200.0 x 200.0  µm²

Sample environments

  • ID30B is equipped with a modified microdiffractometer (MD2-S), allowing data collection from micron-sized crystals mounted on conventional cryo-supports or in crystallisation plates (in situ).
  • A new generation, flexible and high throughput sample changer (FlexHCD) capable of transferring samples from either EMBL/ESRF pucks or Unipucks has also been developed for ID30B in a collaboration between the ESRF and EMBL-Grenoble.

Detectors

  • PILATUS3 6M 1000 µm Si sensor (Dectris). This detector was funded with support from the French Infrastructure for Integrated Structural Biology (FRISBI) ANR-10-INBS-05 to the CNRS and the ESRF.
  • AXAS-A X-ray fluorescence detector (KETEK)

Mueller-Dieckmann, C., Bowler, M. W., Carpentier, P., Flot, D., McCarthy, A. A., Nanao, M. H., Nurizzo, D., Pernot, P., Popov, A., Round, A., Royant, A., de Sanctis, D., von Stetten, D., Leonard, G. A. The status of the macromolecular crystallography beamlines at the European Synchrotron Radiation Facility, Eur. Phys. J. Plus 130, 70 (2015).