EMBO'07 - A one-week course on Exploiting Anomalous Scattering in Macromolecular Structure Determination
18th - 22nd June 2007
ESRF, Grenoble & EMBL Grenoble Outstation - France
ESRF Grenoble Christoph MÜLLER-DIECKMANN, Gordon LEONARD, Didier NURIZZO, Ute RAMSEGER
EMBL Grenoble Outstation Andrew McCARTHY
EMBO Lynne TURNBULL
Scope of the course
This practical course addressed young scientists who intend to apply single- and multiple-wavelength anomalous scattering (SAD & MAD) methods in macromolecular structure determination. The course aimed to impart the theoretical and practical basis for the 3-dimensional structure determination of bio-macromolecules using these techniques. Through a series of lectures, software demonstrations, practicals and tutorials, participants got insights into all aspects of the structure determination process including beamline instrumentation, data collection and processing, heavy atom substructure determination, phasing and model building. Also sessions focusing on automated structure solution procedures and newer methods which exploit small anomalous scattering signals from crystals of native macomolecules have been organised.