In general, a gene which codes for a toxin or a biologically active protein in the physiological mechanisms of the infection or the immunity may be, under certain conditions, a factor of danger whose risk must be examined on a case by case study. The Commission de Génie Génétique (CGG) has established a non-exhaustive list of examples of sequences from categories A and B. The classification into A and B may be modified according to the evolution of knowledge. Concerning toxins; a list is presently being elaboration by the CGG. In the meantime, the CGG will examine case by case the dossiers related to toxins.

Non exhaustive guide of category A and B sequences

I. Viral Sequences

Virus

Sequence

Category

VIH/SIV

tat

B

 

nef

B

 

Vpx

B

 

Vpr

B

 

gag

A

 

pol

A

 

env

A

 

gag , pol , env exprimés simultanément

B

Papillomavirus

E5

B

human and bovine

E5 - HPV

A

 

E5 - BPV

B

 

E6

B

 

E7

B

Rabbit Papillomavirus

E1

B

 

E2

B

 

E6

B

SV 40

T

B

Polyome

LT

A

 

MT

A

 

ST

A

     

The viral sequences capable of modifying the tropism of a virus under certain experimental conditions (surface proteins such as glycoprotein for example) are considered as being in category B

II. Cellular Sequences

Sequences

Category

Growth factors

B(1)

cell cycle factors

B

growth factor receptors

B(1)

hormone receptors

B

interferon a, b, g

B

interleukin 10

A

interleukin (2) 2, 3, 4, 5, 6, 7, 8, 9, 11, 12,

B

interleukin (2) 13, 15, 16

B

viral interleukin 6

B

interleukin 1, a

B

MIF (lymphokine)

B

LIF (leukemia inhibitor factor)

B

RANTES (chemokine)

B

substance P

B

TNF a, b (cytokines)

B

GM-CSF

A

EPO (erythropoïetin) :

- in a transition expression system

- in a prolonged expression system

A

B

HMG-CR

B

MIP 1 a (chemokine)

B

MIP 1 b (chemokine)

B

MIP a 2 (chemokine)

B

SDF 1 a (chemokine)

A

SDF 1 b (chemokine)

A

IP-10 (chemokine)

B

MGF (hématopïétic growth factor)

B

DC-CK1 (chemokine)

B

MDC (chemokine)

B

(1) Adjustment possible after examination of the dossier's arguments

(2) All interleukines except IL 10 are in B

III. Toxins

Toxins are considered as being at least type B sequences. A list of toxins is presently being elaborated by the CGG. In the meantime, the experiments are classified on a case-by-case study according to the potential danger conferred by these sequences.

New list of type A or B inserts

Updated October 2001

   

A

 

B

     

ABC1

 

AkT

b-gal

 

angiostatin

BDNF

 

Bac

GDNF

 

Bad

GPF

 

Bcl

LCAT

 

CK-beta-12

luc

 

CK-beta-9

NT3

 

CNTF

parkin

 

endostatin

presenilin

 

FE65

Protein Kinase

 

FGF

SCA7

 

Gax

scFV

 

MCP1

SREBP

 

p53

TK

 

PPAR d

   

b-amyloid precursor

   

protein G

   

PRP

   

RANTES

   

TGFb (BMP; CDMP)

   

VEG1

   

VEGF

   

vIL10

Translation from the French Genetic Engineering Commission Guide