Aim of this protocol: To help the LC startup the beamlines quickly and efficiently. Please use the log book to note current readings and anomalies.

## Write down in the beamline logbook the following information :

• Date
• Experiment number (e.g. MX-415)
• Your phone number on site and off-site, may also be written on white board.

## Inside the experimental hutch :

• Check that the hutch is clean (if not note this in the log book and mail to BLSci)
• Check that all tools are in/on tool box/board
• Gloves and glasses are available to handle liquid N2.
• Glasses for laser protection.
• Check that no sample is on the goniometer
• Check that the cryo stream is aligned and working, level of N2 is ok,  temperature regulation @ 100K
• Put the YAG screen on the goniometer (except ID23eh2 and ID29)
• Check that mxCuBE_hutch is working - use it to align the YAG screen.
• Close the experimental hutch.

## Align the beamline :

• Check that all applications needed on the end-station are running :
• Bliss device servers
• Front end and Vacuum applications
• Spec oh
• Spec eh
• mxControlPanel
• Firefox and ISPyB
• Do a Realign of the beamline (except ID23-1 -> use LC_startup in eh1).
• In the logbook write  (in addition ID23-1 -> use LC_startup in eh1) :
• Machine status : ring current and operation mode.
• Check Front end is open.
• Check that automatic mode will last the experiment time.
• The energy if tuneable beamline
• Undulator Gaps
• The values of the diodes along the beam path with all shutters open and 100% transmission and slits open to beamline standard sizes (note also the gain)
• Check that these values fit the standards of the beamline (compare at least with values of the day before).
• With the safety shutter and fast shutter open, check that beam is centered.

## Verify data collection with user account:

• Collect with mxCuBE at 1.7Å a blank image, should be free of beamstop artefacts.
• If test Xtals use one to collect images with mxCuBE at 1.7Å and to test EDNA (mxCuBE EDNA tab)
• Check that ISPyB has been updated.
• Check that data processing input files (XDS.INP, MOSFLM.INP,etc...) are created and that the automatic data processing is launched.

## Sample changer:

• Check that the gonio head is the Smart Magnet head or miniKappa.
• Check that there is no baskets in the sample changer.
• If not tested before, put a test basket (with test samples) then test the sample changer (load/unload sample) at least 5 different samples using mxCuBE.

## Safety and other stuff with users presence:

• Check that users have done their safety training and are aware of a proper use of the PSS system.
• Explain about the oxygen level alarm and what to do if it sounds.
• Check that users know how to shut and open the experimental hutch door.
• Check that users are confident with using the beamline.
• Leave phone numbers where you can be contacted at all times (from 8 am until 10 pm).

A couple of hours after the last data processing file is written to a session in a user account, the ownership of all the experiement files are changed to protect the user's data.

Only the user of concern and ESRF staff can access to them.

For the later, you need to add a public SSH key to the protected proposal account. In order to do that, follow the instructions at http://dch.esrf.fr. Then, from a rnice6 terminal opened from your own account, you can login as your user by typing ssh –X user_name@rnice6.

If you don't have added ssh keys before you first have to type the command ssh-keygen from your nice account.




## For ID23-2 only

• Note in logbook pressure on the KB vessel  - check pressure sensor (blue device at the left side of the KB vessel) (985-1000 mbar).
• Check that helium cylinder (behind the optics hutch electronic racks).

(If empty contact John Surr (working hours) or the floor operator (25 25)