Local Contact Startup Guidelines.

Aim of this protocol: To help the LC startup the beamlines quickly and efficiently. Please use the log book to note current readings and anomalies.

Write down in the beamline logbook the following information :

• Date
• Experiment number (e.g. MX-415)
• Your name
• Your phone number on site and off-site, may also be written on white board.

Inside the experimental hutch :

• Check that the hutch is clean (if not note this in the log book and mail to BLSci)
• Check that all tools are in/on tool box/board
• Gloves and glasses are available to handle liquid N2.
• Glasses for laser protection.
• Check that no sample is on the goniometer
• Check that the cryo stream is aligned and working, level of N2 is ok,  temperature regulation @ 100K
• Put the YAG screen on the goniometer (except ID23eh2)
• Check that exes_hutch is working - use it to align the YAG screen.
• Close the experimental hutch.

Align the beamline :

• Check that all applications needed on the end-station are running :
• Bliss device servers
• Front end and Vacuum applications.
• Spec oh
• Spec eh
• mxControlPanel (Id23-1,2 and Id29)
• mxCuBE : log in as users
• Firefox and ISPyB
• restart DNA (after logging into mxCuBE)
• Image viewer
• Do a Realign of the beamline (except ID23-1 -> use LC_startup in eh1).
• In the logbook write  (in addition ID23-1 -> use LC_startup in eh1) :
• Machine status : ring current and operation mode.
• Check Front end is open.
• Check that automatic mode will last the experiment time.
• The energy if tuneable beamline
• Undulator Gaps
• The values of the diodes along the beam path with all shutters open and 100% transmission and slits open to beamline standard sizes (note also the gain)
• Check that these values fit the standards of the beamline (compare at least with values of the day before).
• With the safety shutter and fast shutter open, check that beam is centred.

Verify data collection with user account:

• Collect with mxCuBE at 1.7Å a blank image, should be free of beamstop artefacts.
• If test Xtals use one to collect images with mxCuBE at 1.7Å
• Same thing with DNA
• Check that ISPyB has been updated.

Sample changer:

• Check that the gonio head is the Smart Magnet head or miniKappa.
• Check that there is no baskets in the sample changer.
• If not tested before, put a test basket (with test samples) then test the sample changer (load/unload sample) using mxCuBE.

Safety and other stuff with users presence:

• Check that users have done their safety training.
• Explain about the oxygen level alarm and what to do if it sounds.
• Check that users know how to close the experimental hutch door.
• Check that users are confident with using the beamline.
• Check that users know the door code.
• Leave phone numbers where you can be contacted at all times (until 10 pm).
  

For ID23-2 only

• Note in logbook pressure on the KB vessel  - check pressure sensor (blue device at the left side of the KB vessel) (985-1000 mbar).

• Check that helium cylinder (in the optics hutch electronic rack).

(If empty contact John Surr (working hours) or the floor operator (25 25)