How to use our beamlines
These page contain all the information required to complete a successful experiment at the Macromolecular Crystallography Group beamlines of the ESRF.
Before you come to the ESRF please read the "Prepare Your Experiment" pages. Once here the "Run Your Experiment" pages will help you along the way and if you get into any difficulties the "Trouble Shooting" pages should have the answers. If you are struggling to find information then please use the "MX Search Engine".
All of our beamlines are equipped with a high precision minidiffractometer and a sample changer (for which you must use SPINE standard pins).
Please cite the paper describing MXCuBE for experiments conducted at the ESRF Structural Biology Beamlines, Gabadinho, J., et al. (2010).MxCuBE: a synchrotron beamline control environment customised for macromolecular crystallography experiments. J. Synch. Rad., 17, 700-707
View structures deposited in the Protein Data Bank (PDB) solved using the ESRF MX Beamlines.
| Beamline | Energy Range | Typical Beamsize (microns) | Resolution Limits | Typical exposure Time | Maximum recommended phi speed | Number of passes | Typical transmission for MAD/SAD | |
|---|---|---|---|---|---|---|---|---|
|
ID14-1 |
0.934Å (13.274keV) |
100 |
0.95Å |
1-5 sec |
0.3s/degree |
3 |
Usually 100% |
|
|
ID14-2 |
0.933Å (13.289keV) |
100 |
1.0Å |
1-5 sec |
0.3s/degree |
3 |
Usually 100% |
|
|
ID14-4 MAD |
0.85 to 1.3Å |
100 |
0.88Å |
0.2 - 1 sec |
0.3s/degree |
1 |
10-20% |
|
|
ID23-1 |
0.62 to 2.5Å (20 to 5.0keV) |
50 |
0.64Å |
0.1 - 1 sec |
0.1s/degree |
1 |
1-5% |
|
|
ID23-2 |
0.873Å (14.200keV) |
10 |
0.90Å |
1 sec |
0.1s/degree |
1 |
5-20% |
|
|
ID29 |
0.62 to 2.4Å (20 to 5.2keV) |
60 |
0.64Å |
0.1 - 1 sec |
0.1s/degree |
1 |
1-5% |
|
|
BM29 |
0.83 to 1.77Å (15 to 7 keV) |
500 |
1nm |
1 sec |
no rotation |
10 frames |
Usually 100% |