I.      Specifications of the Raman spectrometer

• Fluorescence and photochemical modification of biological samples by laser beam minimized from 650 to 800 nm : Laser source NIR 785nm
• Raman scattering of biological samples is low: Power at the sample > 50mW
• Spectral range from 200 cm^-1 to 4000 cm^-1 with a resolution < 1 cm^-1
• A compact probe (back scattering mode) compatible with X-ray goniometer
• Classical crystal size down to 50 μm: exaltation focal spot 50 μm

II.      Raman equipment at the cryobench

III.      Raman compact probe, integration in the microspectrophotometer

Microspectrophotometer	Support of probe

Sample environnement

IV.      An example: Raman spectra of Tetragonal Lysozyme Single Crystals

1. Raman spectra as published in: A. B. Kudryavtsev et al. “Polarized Raman Spectroscopic Studies of Tetragonal Lysozyme Single Crystals” Acta Crysallographica (1998). D54, 1216

2. Unpolarized Raman Spectrum of Tetragonal Lysozyme Single Crystal recorded from 400 cm-1 to 2000 cm-1 at the cryobench with an acquisition time of 5 mins It is obvious (red square) that this spectrum is similar to the one in 1.a)